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Rate of Killings Unabated. The authorities say they ordered the emergency decree, imposed on June 12, to guard against what the Government calls a ''revolutionary onslaught'' from black radicals. Since the decree was proclaimed, however, people have been killed in black townships, by official count.
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The current rate of killing - roughly people a month - seems as high as at any time in the last two years. The authorities also announced that the police shot dead four African National Congress insurgents near the border with independent Swaziland, and that four insurgents from the South West Africa People's Organization were killed near the Angolan border after they launched a mortar attack on the town of Oshakati, 40 miles south of the frontier.
Three civilians died in the attack, the authorities said.
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The South West Africa People's Organization, based in Angola, has been fighting a low-level guerrilla war for two decades, seeking to end Pretoria's control of South West Africa, a former German colony also known as Namibia. The territory's economy, built on uranium and diamond mining, is tightly linked with that of South Africa, and South African officials there have said economic sanctions against South Africa would also spill into the territory.
Internal Debate on Sanctions. The debate over sanctions has become a central issue here, with the authorities bluntly challenging the outside world to impose the sanctions,and the Government's critics urging that the measures be imposed so as to speed the end of the policies of apartheid. Site pharmacists were aware of the randomization assignments to ensure proper handling and dispensing, which included application of overlays to all syringes for blinding before delivery to site staff; Biojector delivery did not require an additional step to ensure blinding.
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C and All assays were performed blinded in HVTN laboratories utilizing validated methods [ 5 , 26 — 28 ]. Details of the antigens used for the antibody and cellular assays are listed in S1 Table. The mer peptide pools corresponded to the following vaccine inserts: Env. C, Env. For positive response criteria, see S1 Text.
For antigen and positive response criteria descriptions, see S1 Text. This included the 3 vaccine-derived strains 96ZM C, both tier 2 [moderate sensitivity to antibody-mediated neutralization] [ 30 ] and TV1c8. C, a tier 1A as well as MW C, a tier 1A virus. If a titer was left-censored, half the left limit was used as the titer value.
A response was considered positive if the neutralization titer was above 10 one-half the lowest dilution tested. All analyses were prespecified and based on the per-protocol cohorts of HVTN and HVTN , which consisted of all participants who received the first 4 scheduled vaccinations see S2 Text. Participants who had a positive test for HIV-1 by month 6. The 2 vaccine arms of HVTN i. Bar charts were used to illustrate the positive response rates and boxplots to show the distributions of immune responses to individual antigens or pseudoviruses at the peak immunogenicity time point, month 6.
Immune responses were summarized by the proportion of participants with a positive response rate and by magnitude geometric mean titer [GMT] for antibody assays, percentage T cells expressing marker combination for cellular assay. Super learning and targeted minimum loss estimation TMLE [ 34 ] were used as the primary analysis to estimate and compare mean immune response rates and mean magnitudes overall and among positive responders, adjusting for possible confounding by age, sex, and body mass index BMI.
All p -values are two-sided, with p -values less than 0. For each distinct hypothesis, the number of multiple tests was limited, and therefore multiplicity adjustments were not made. SAS version 9. Between June 21, , and July 13, , participants from 3 sub-Saharan African countries were enrolled in HVTN , of whom 30 were allocated to each of the 4 vaccine arms and 6 allocated to each of the 2 placebo arms.
All vaccinations were safe and well tolerated [ 22 , 25 ].
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Of those enrolled in the HVTN DNA plasmid prime arms of interest [i] and [ii] above , 29 and 27 participants, respectively, were in the per-protocol cohort; were in the HVTN vaccine per-protocol cohort. No positive responses were seen among placebo recipients in either trial data not shown. B to C V1V2 S2 Table. Orange font indicates the vaccine-matched antigens.
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A ZM C gp, B C gp, and C TV1c8. C gp Bar charts show the observed, unadjusted positive response rates. Boxplots show observed responses and are based on positive responders only shown as colored circles. The response rate to C V1V2 and 3. B S3 Table. Boxplots show observed responses and are based on positive responders only shown as colored circles , negative responders are shown as grey triangles.
C and MW C pseudoviruses: No responses were seen to the tier 2 subtype C vaccine strains 96ZM C, The highest response rate was to Env. C, at This was followed by response rates to Env. C of Rates to Env. C were The magnitude of responses to the vaccine-matched Gag-LAI among 3 HVTN vaccine recipients with positive responses was very high Fig 5 , and these highest 3 participants had strong responses to Env antigens as well.
No other significant differences were seen between these 2 arms. Our cross-protocol analysis revealed several important findings.
First, DNA plasmid priming followed by protein boosting led to increased antibody and cellular immune responses when compared to a canarypox virus prime followed by matched protein boosting. Finally, canarypox virus priming was not superior to DNA plasmid priming for any of the immune responses we examined. DNA plasmid vaccines have traditionally been considered less immunogenic than viral vectors, but our findings suggest that this paradigm should be reevaluated. Compared with earlier DNA plasmid vaccine studies, improvements in vector design of these DNA plasmid vaccine constructs such as more efficient promoter and regulatory elements, RNA secondary structure modification, and codon optimization of the insert [ 35 , 36 ] may have resulted in either increased protein expression or expressed protein that is inherently more immunogenic.
The higher antibody responses observed suggest that increased in vivo protein expression by the newer DNA plasmid vaccine vector may be the more likely explanation, as higher protein dose correlates with antibody titers and response rates with gp Env vaccination [ 37 ] and other vaccines [ 38 , 39 ].
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As other studies have shown Biojector delivery of DNA plasmid vaccines to improve cellular and antibody immunogenicity [ 23 , 24 , 41 ], this appears to be a contributory factor in the increased cellular immunogenicity that we observed in the Biojector DNA plasmid primed arm compared to the ALVAC primed arm. People Who Just Had Sex. Party Legends. For the first in our Worn In series, we travel to downtown Johannesburg, where for the Skhothane, life is all about looking good and making money.
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